Abstract
A fluorescent in situ hybridization (FISH) technique, based on binding of a fluorescein-labelled oligonucleotide probe to rRNA, was used to determine a viable population of bacteria. We designed the oligonucleotide probes targeted to 16S rRNA of anaerobic beer-spoilage bacteria belonging to the genus Pectinatus and assessed the sensitivity and specificity of the FISH method using the bacteria spiked into beer. The FISH technique could be applied directly to the beer samples without a culturing step, which typically requires several days. This method was able to detect beer-spoilage bacteria in a species-specific manner within 5 hr. In addition, we developed another useful application of the FISH, a quantitative method using an internal control for enumeration.
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