Abstract
We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.
Highlights
Rickettsia raoultii belongs to the spotted fever group rickettsiae and can cause Dermacentor-borne necrotic erythema and lymphadenopathy (DEBONEL), tick-borne lymphadenopathy (TIBOLA), and scalp eschar and neck lymphadenopathy (SENLAT) in humans [2]
Anaplasma, Babesia, Borrelia, Neoehrlichia, Rickettsia, and Theileria present within the study we obtained an extensive impression of tick-borne pathogens of the genera Anaplasma, area
As the molecular identification and/or confirmation of Ixodes ricinus ticks remained and/or confirmation of to Ixodes ricinus remained we refer theseticks, ticks inconclusive, we refer these ticksticks as part of the inconclusive, I. ricinus complex
Summary
R. raoultii,disease, are considered emerging pathogens with ettsiosis is often an underdiagnosed and many aspectstick-borne are neglected [8] With the their vector expanding to novel loci worldwide, in raised. Collected the reverse a large panel of tick-borne including raoultii ticks strainusing This enabled us toimpression simultaneously screen forpathogens the presence of agenera large this blot method, obtained an extensive of tick-borne of the panel of tick-borne microorganisms, including. Using this method, Anaplasma, Babesia, Borrelia, Neoehrlichia, Rickettsia, and Theileria present within the study we obtained an extensive impression of tick-borne pathogens of the genera Anaplasma, area. Borrelia, Neoehrlichia, Rickettsia, and Theileria present within the study area
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