Novel Protein Binding Capacity and Selectivity of Immobilized Pyridinium Pseudo-Affinity Gels: Applications in Immunoglobulin G Purification and Quantitative Determination

Abstract

Abstract A series of novel pseudo-affinity gels using synthetic, immobilized pyridinium ligands have been prepared. These gels exhibit extraordinarily high protein binding capacity and selectivity. Initial applications of these gels were directed toward the purification and quantitative, rapid determination of immunoglobulins. The results showed that these pseudo-affinity gels functionally mimic, to a high degree of similarity, the behavior of immobilized Protein A or G for immunoglobulins purification. In contrast to the labile nature of protein-based affinity gels, these immobilized pyridinium gels can withstand harsher treatments, such as prolonged exposure of the gels to proteolytic enzymes, organic solvents, acid or base and high temperatures.