Abstract
The potentiometric sensing device for quantitative estimation of creatinine is reported. The sensor comprises a bi-enzymatic reactor and potentiometric urea biosensor. The bi-enzymatic reactor is developed by sandwiching two enzymes, creatininase and creatinase, within two layers of organically modified sol–gel glass (ORMOSIL). The urea biosensor is developed by immobilizing urease on a new pH sensor developed by p-toluenesulfonate doped polyaniline-modified electrode. The polyaniline-modified electrode is developed by potentiodynamic electropolymerization of aniline based on sweeping the electrode potential within −0.7 to 2.0 V versus Ag/AgCl in non-aqueous medium in the presence of tetraethylammonium p-toluenesulfonate as supporting electrolyte. The enzyme reactor converts creatinine into urea. The urea formed through bienzymatic reactor is monitored by new urea biosensor. The typical results on urea sensing, pH sensing and creatinine sensing are reported. The detection limit of the new sensor for urea is 5 μM and for creatinine is 100 μM.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
