Novel polymorphic microsatellite markers for population genetics of the endangered Caribbean star coral, Montastraea faveolata

Abstract

Montastraea faveolatais a reef-building Caribbean coral that is currently listed as endangered across its range. A better understanding of the population genetic structure, ge- netic diversity and connectivity is needed to make sound conservation plans for this species. Here, we describe nine novel polymorphic microsatellite loci mined from currently available sequence data. Loci were screened in two widely separated populations (n021 individuals per population) from the Flower Garden Banks (northern Gulf of Mexico) and Curacao (Netherland Antilles, southern Caribbean). Allelic diversity ranged from 3 to 16 and observed heterozygosities ranged from 0.095 to 0.905. For all loci but one, the Hardy- Weinberg equilibrium hypothesis wasnot rejectedwithineach population. These loci failed to amplify symbiont DNA iso- lated from pure Symbiodinium cultures, confirming their coral-specificorigin. We also describea multiplexing protocol for these markers reducing the costs and time required for future genetic studies. Finally, all markers were tested in the two sister species, M.franksiandM.annularis,and successful amplification and polymorphism were confirmed. The marker panel reportedhere, incombination with previously published markers for the samespecies complex,willfacilitate coral reef connectivity research for this ecologically important genus, Montastraea, across the Caribbean.