Novel plant flavonoid electrochemical sensor based on in-situ and controllable double-layered membranes modified electrode.

Jing Hu,Xin Yang,Feilong Hu,Hongwei Lin,Renjie Zhou,Qiuyuan Wei,Shabi Abbas Zaidi

doi:10.1371/journal.pone.0237583

Jing Hu, Xin Yang + Show 5 more

Open Access

https://doi.org/10.1371/journal.pone.0237583

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Abstract

Identification and quantification of plant flavonoids are critical to pharmacokinetic study and pharmaceutical quality control due to their distinct pharmacological functions. Here we report on a novel plant flavonoid electrochemical sensor for sensitive and selective detection of dihydromyricetin (DMY) based on double- layered membranes consisting of gold nanoparticles (Au) anchored on reduced graphene oxide (rGO) and molecularly imprinted polymers (MIPs) modified glassy carbon electrode (GCE). Both rGO-Au and MIPs membranes were directly formed on GCE via in-situ electrochemical reduction and polymerization processes step by step. The compositions, morphologies, and electrochemical properties of membranes were investigated with X-ray powder diffractometry (XRD), Fourier transform infrared spectrum (FTIR), Field emission scanning electron microscopy (FESEM) combined with various electrochemical methods. The fabricated electrochemical sensor labeled as GCE│rGO-Au/MIPs exhibited excellent performance in determining of DMY under optimal experimental conditions. A wide linear detection range (LDR) ranges from 2.0×10−8 to 1.0×10−4 M together with a low limit of detection (LOD) of 1.2×10−8 M (S/N = 3) were achieved. Moreover, the electrochemical sensor was employed to determine DMY in real samples with satisfactory results.

Highlights

Dihydromyricetin (DMY) is the most bioactive and abundant plant flavonoid found in the leaves of Ampelopsis grossedentata
The (002) peak at 2θ = 26 ̊ exhibits the crystalline feature of Gr, indicating the formation of reduced graphene oxide (rGO) via an electrochemical reduction of graphene oxide (GO) (Fig 2b) [27]
The results demonstrated that double-layered membranes of rGO-Au and molecularly imprinted polymers (MIPs) have been step by step immobilized on glassy carbon electrode (GCE)

Summary

Introduction

Dihydromyricetin (DMY) is the most bioactive and abundant plant flavonoid found in the leaves of Ampelopsis grossedentata (a traditional herbal tea in Huaihua region of China and its alternative name is vine tea). To the best of our knowledge, there are no reports based on neither rGO-Au nanocomposite nor MIPs modified electrode to quantitative analysis of DMY till now. Based on our previous researches in Gr-based nanocomposite and MIPs [10, 11, 18], this investigation aims at fabricating a novel DMY electrochemical sensor based on double-layered membranes consisting of rGO-Au nanocomposite and MIPs modified GCE (labeled as GCE│rGO-Au/MIPs).

Results

Conclusion