Novel plant and fungal AGP-like proteins in the Medicago truncatula–Glomus intraradices arbuscular mycorrhizal symbiosis

Abstract

The ability of arbuscular mycorrhizal (AM) fungi to colonise the root apoplast, and in coordination with the plant develop specialised plant-fungal interfaces, is key to successful symbioses. The availability of expressed sequence tags (EST) of the model legume, Medicago truncatula, and AM fungus, Glomus intraradices, permits identification of genes required for development of symbiotic interfaces. The M. truncatula EST database was searched to identify cell surface arabinogalactan-proteins (AGPs) expressed in mycorrhizal roots. Candidate genes were characterised and gene expression tested using reverse transcription polymerase chain reaction and promoter:reporter gene fusions. Genes encoding one plant AGP and three AGP-like (AGL) proteins (from G. intraradices) were identified. AGL proteins encoded by two AGL genes from G. intraradices (GiAGLs) represent a new structural class of AGPs not found in non-AM fungi or plants. Two GiAGLs differ from plant AGPs by containing charged repeats. Structural modelling shows that GiAGL1 can form a polyproline II helix with separate positively and negatively charged faces, whereas GiAGL3 is charged on all three faces. The unique structural properties of the newly discovered AGLs suggests that they could assist the formation of symbiotic interfaces through self-assembly and interactions with plant cell surfaces.