Abstract
In this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5’UTRIRES-?/P1(VP0-VP3-VP1)/P2(2A1NPG↓P-2A2H-box/NC-2B-2C)/P3(3A-3BVPg-3CPro-3DPol)/3’UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae.
Highlights
Picornaviruses are small non-enveloped viruses with a positive-sense, single-stranded RNA genome, belonging to the family Picornaviridae
We report the identification and complete genome characterization of a potentially novel picornavirus in freshwater fishes (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) in Hungary
Six official fishorigin genera of picornaviruses (Limnipivirus, Potamipivirus, Fipivirus, Symapivirus, Rajidapivirus, and Danipivirus) have been established, but several picornaviruses identified in saltwater and freshwater fishes are waiting for their official taxonomy to be determined [20]
Summary
Picornaviruses are small non-enveloped viruses with a positive-sense, single-stranded RNA genome, belonging to the family Picornaviridae. The family consists of 158 species grouped into 68 genera (https://www.picornaviridae.com/index.html). Is the known genetic diversity of the picornaviruses expanding rapidly but the number of known host species is increasing. Picornaviruses have been identified in a wide range of vertebrates from fish to mammals, including humans. Most of the known picornaviruses were found in mammals and birds, but in recent years, novel. Nucleotide sequence data reported are available in the DDBJ/ EMBL/GenBank databases under the accession number MW590713
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