Abstract
The novel pathways of oleic acid formation from cis-vaccenic ( cis-11-octadecenoic) acid and of cis-vaccenic acid formation from oleic acid by enzymatic positional isomerization have been proposed in higher plants, based on stable-isotope experiments using[2,2- 2H 2] cis-vaccenate or [2,2- 2H 2]oleate as an immediate precursor. A pulp homogenate and also pulp slices prepared from developing kaki ( Diospyros kaki) fruit could catalyze these hitherto unknown isomerizations. This suggests the presence of a new type of isomerase responsible for the double-bond shifting reaction without cis- trans isomerization in the middle of fatty acid carbon chains.
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