Abstract
Circulating levels of oestrogen are known to modulate autonomic function via the conventional nuclear oestrogen receptors (ERα and ERβ) localised in several areas of the brain. Recently several lines of evidence suggest that the orphan G protein-coupled receptor GPR30 may act as a cytoplasmic oestrogen receptor. This study was designed to examine the expression and localisation of GPR30 in the brains of male and female rats using immunohistochemistry on fixed brain sections with a rabbit antibody to GPR30 and reverse transcription polymerase chain reaction (RT-PCR) on tissue punches.
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