Novel N3-Schiff base and its zinc halide/pseudohalide complexes: Antimicrobial, antioxidant, cytotoxicity and burn wound healing activities

Abstract

A new N3-tridentate Schiff base ligand(L) was synthesized by condensation reaction of 2-methoxycinnamaldehyde and diethylenetriamine. Thereafter some new zinc complexes formulated as [ZnLX2] (X is halide or pseudo-halide) were prepared using this chelating ligand. The suggested structures of these compounds were identified by melting point and molar conductivity measuring, UV–Visible, FT/IR, 1H and 13C NMR and MASS spectral analyses. Moreover, the thermal behaviors of the ligand and zinc complexes were investigated under N2 atmosphere and at a heating rate of 20 °C/min. Zinc bromide and chloride complexes were synthesized in nanostructured sizes by sonochemical method and used as precursor to prepare nanostructured ZnO approved by SEM, EDX and XRD analyzes. Furthermore antibacterial, antifungal and antioxidant properties were evaluated by well diffusion, ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) methods respectively. Cytotoxicity properties of the ligand and zinc complexes were assessed against prostate cancerous cell lines (PC3) as compared with HUVEC normal cells. The morphology of PC3 cancer cells treated by the ligand and some zinc complexes were also visualized by images. Eventually, burn wound healing activities of the ligand and zinc chloride complex were examined for healing of the second degree burns in the rats in a period of 20 days.