Abstract
Based on N-doped carbon dots/β-cyclodextrin nanocomposites modified glassy carbon electrodes (N-CDs/β-CD/GCE), an effective electrochemical sensor for enantioselective recognition of tryptophan (Trp) enantiomers was developed by differential pulse voltammograms (DPVs). Fluorescent N-CDs were synthesized through a hydrothermal method and characterized by spectroscopic approaches. The N-CDs/β-CD nanocomposites were efficiently electrodeposited on the surface of GCE through C–N bond formation between N-CDs and electrode. The obtained N-CDs/β-CD/GCE was characterized by multispectroscopic and electrochemical methods. Such N-CDs/β-CD/GCE generated a significantly lower Ip and more negative Ep in the presence of l-Trp in DPVs, which was used for the enantioselective recognition of Trp enantiomers. The N-CDs/β-CD nanocomposites showed different binding constants for tryptophan enantiomers, and they further selectively bonded with l-Trp to form inclusion complexes. This N-CDs/β-CD/GCE combined advantages of N-CDs with strong C–N binding ability and β-CD with specific recognition of Trp enantiomers to fabricate a novel sensing platform for enantioselective recognition of Trp enantiomers. This strategy provided the possibility of using a nanostructured sensor to discriminate the chiral molecules in bio-electroanalytical applications.
Highlights
The enantiomeric forms of chiral drugs usually show different pharmacological/metabolic behaviors in biological systems, and the demand for single-enantiomer drugs has substantially increased throughout the world, so the enantioselective recognition of enantiomeric forms of chiral molecules has attracted huge attention in the field of analytical and pharmaceutical research [1,2,3,4]
N-carbonthe dots (CDs)/β-cyclodextrin of (β-CD)/glassy carbon electrodes (GCE) to L-Trp or D-Trp in 10 mM PB were carefully investigated by differential pulse voltammograms (DPVs) with instrumental parameters as: potentials range from −0.1 to 0.6 V; pulse amplitude 0.05 V; pulse width
It is clear that N-CDs exhibit broad absorption bands from 200 nm to 500 nm with an obvious UV-vis absorption peak centered at 282 nm
Summary
The enantiomeric forms of chiral drugs usually show different pharmacological/metabolic behaviors in biological systems, and the demand for single-enantiomer drugs has substantially increased throughout the world, so the enantioselective recognition of enantiomeric forms of chiral molecules has attracted huge attention in the field of analytical and pharmaceutical research [1,2,3,4]. Have reported the electrochemical recognition of Trp isomers by using β-CD or copper-modified carbonthe dots (CDs) have attracted considerable attention in biological areas due to β-CDFluorescent [15,16,17], proving possibility of β-CD-based electrochemical enantioselective recognition. (N-CDs)defects draw great attention amongthe researchers sinceproperty nitrogen of doping can effectively there are the almost relative on N-CDs-based enantioselective of Trp modulate surfacenodefects and reports greatly enhance the photoelectron property ofrecognition. Multi-walled carbon these nanotube andsuggest graphene feasibilitydots of N-CDs-based enantioselective recognition of Trp enantiomers. Quantum all belong to electrochemical the family of carbon nanomaterials, these reports suggest the feasibility of Inspired by these facts, we first report a novel electrochemical nanocomposite based on N-CDs. N-CDs-based electrochemical enantioselective recognition of Trp enantiomers.
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