Novel Mononuclear Ruthenium(II) Compounds in Cancer Therapy

Shyam Sunder Anchuri,Sreekanth Thota,Satyavati Dhulipala,Rajeshwar Yerra,Krishna Prasad Devarakonda

doi:10.7314/apjcp.2012.13.7.3293

Shyam Sunder Anchuri, Sreekanth Thota + Show 3 more

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https://doi.org/10.7314/apjcp.2012.13.7.3293

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Abstract

The present study was conducted to evaluate in vivo anticancer activity of two novel mononuclear ruthenium(II) compounds, namely Ru(1,10-phenanthroline)2(2-nitro phenyl thiosemicarbazone)Cl2 (Compound R1) and Ru (1,10-phenanthroline)2(2-hydroxy phenyl thiosemicarbazone)Cl2 (Compound R2) against Ehrlich ascites carcinoma (EAC) mice and in vitro cytotoxic activity against IEC-6 (small intestine) cell lines and Artemia salina nauplii using MTT [(3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide)] and BLT [brine shrimp lethality] assays respectively. The test ruthenium compounds at the doses 2 and 4 mg/kg body weight showed promising biological activity, especially in decreasing tumor volume, viable ascites cell counts and body weights. These compounds prolonged the life span (% ILS), mean survival time (MST) of mice bearing-EAC tumor. The results for in vitro cytotoxicity against IEC-6 cells showed the ruthenium compound R2 to have significant cytotoxic activity with a IC50 value of 20.0 μg/mL than R1 (IC50=78.8 μg/mL) in the MTT assay and the LC50 values of R1 and R2 compounds were found to be 38.3 and 43.8 μg/mL respectively in the BLT assay. The biochemical and histopathological results revealed that there was no significant hepatotoxicity and nephrotoxicity associated with the ruthenium administration to mice.

Highlights

For the survival of any organism, there should be a delicate balance between the cell growth and death
In vitro cytotoxicity assay: The in vitro cytotoxic activity of ruthenium compounds was done using Brine shrimp lethality (BLT) assay against Artemia salina nauplii and MTT assay against IEC-6 cell lines
Our results stated that R1 &R2 compounds increased tissues were subjected to histopathological examination the lifespan of Ehrlich ascites carcinoma (EAC) bearing mice only at higher in order to assess the alterations in these parameters of the concentrations (P

Summary

Introduction

For the survival of any organism, there should be a delicate balance between the cell growth and death This balance can get disturbed in a number of ways, which may lead to either abnormal growth of tissue (Scott, 2003) or may develops into a lethal tumor or cancer (Folkman and Kalluri, 2004). Chemotherapy is an established course of therapy to treat malignancies but the most of the currently available drugs are not specific to tumor cells. That is, they damages the normal tissues that proliferate rapidly (bone marrow, hair follicles and intestinal epithelium), which often limits their usefulness (Brunton et al, 2005)

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