Abstract

Methicillin-resistant Staphylococcus remains a severe public health problem worldwide. This research was intended to identify the presence of methicillin-resistant coagulase-negative staphylococci clones and their staphylococcal cassette chromosome mec (SCCmec)-type isolate from patients with haematologic diseases presenting bacterial infections who were treated at the Blood Bank of the state of Amazonas in Brazil. Phenotypic and genotypic tests, such as SCCmec types and multilocus sequence typing (MLST), were developed to detect and characterise methicillin-resistant isolates. A total of 26 Gram-positive bacteria were isolated, such as: Staphylococcus epidermidis (8/27), Staphylococcus intermedius (4/27) and Staphylococcus aureus (4/27). Ten methicillin-resistant staphylococcal isolates were identified. MLST revealed three different sequence types: S. aureus ST243, S. epidermidis ST2 and a new clone of S. epidermidis, ST365. These findings reinforce the potential of dissemination presented by multi-resistant Staphylococcus and they suggest the introduction of monitoring actions to reduce the spread of pathogenic clonal lineages of S. aureus and S. epidermidis to avoid hospital infections and mortality risks.

Highlights

  • Methicillin-resistant staphylococci stand as main pathogens responsible for high levels of infectious processes worldwide

  • Methicillin resistance is encoded by the mecA gene, located at the staphylococcal cassette chromosome mec (SCCmec)

  • In Staphylococcus aureus, types I, VII and XI are recognised, whereas types IV and V are found in Staphylococcus epidermidis and Staphylococcus haemolyticus (Strandén et al 2009, Iorio et al 2012)

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Summary

Introduction

Methicillin-resistant staphylococci stand as main pathogens responsible for high levels of infectious processes worldwide. Methicillin resistance is encoded by the mecA gene, located at the staphylococcal cassette chromosome mec (SCCmec).

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