Abstract
In this work, a kind of novel redox-active species, metal-organic nanocomposites with uniform morphology and good conductivity, was synthesized by one-pot method using HAuCl4 as oxidant and methylene blue as monomer. With excellent biocompatibility of Au, the immunosensing probe was fabricated by means of immobilization of antibody on the nanocomposites for the purpose of signal amplification. Meanwhile, graphene-Au modified electrode was used as the substrate. A new electrochemical immunosensing platform was designed and simply fabricated. With the use of α-fetoprotein (AFP) as a model analyte, good performance was obtained with a wide linear range from 1pgmL−1 to 100ngmL−1, and low detection limit of 19.6fgmL−1. The assay results of clinical serum with this immunoassay were well consistent with those obtained from ELISA method. The present work suggested an electrochemical immunosensing platform for tumor biomarker.
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