Abstract
With an objective of mapping novel low soil P (Phosphorus) tolerance loci in the non-Pup1 type donor rice line, Wazuhophek, we screened a recombinant inbred line (RIL) mapping population consisting of 330 lines derived from the cross Wazuhophek x Improved Samba Mahsuri (which is highly sensitive to low soil P) in a plot with low soil P for tolerance associated traits. Molecular mapping with SSR markers revealed a total of 16 QTLs (seven major and nine minor QTLs), which are associated with low soil P tolerance related traits. Interestingly, a QTL hotspot, harbouring 10 out of 16 QTLs were identified on the short arm of chromosome 8 (flanked by the makers RM22554 and RM80005). Five major QTLs explaining phenotypic variance to an extent of 15.28%, 17.25%, 21.84%, 20.23%, and 18.50%, associated with the traits, plant height, shoot length, the number of productive tillers, panicle length and yield, respectively, were located in the hotspot. Two major QTLs located on chromosome 1, associated with the traits, total biomass and root to shoot ratio, explaining 15.44% and 15.44% phenotypic variance, respectively were also identified. Complex epistatic interactions were observed among the traits, grain yield per plant, days to 50% flowering, dry shoot weight, and P content of the seed. In-silico analysis of genomic regions flanking the major QTLs revealed the presence of key putative candidate genes, possibly associated with tolerance.
Highlights
Rice is one of the world’s most important and principal cereal crops and more than half of the global population derive their calorific requirements from it
Significant phenotypic variation has been observed in rice for different traits related to productivity under low soil P conditions, indicating the possibility of increasing rice production in P poor soils [18,19,20]
Pup1 is the only major QTL associated with low soil P tolerance identified so far and there is an imminent need to identify additional, novel, non-Pup1 type QTLs associated with low P tolerance in rice
Summary
Rice is one of the world’s most important and principal cereal crops and more than half of the global population derive their calorific requirements from it. A major QTL associated with low soil P tolerance, named Pup explaining upto 80% phenotypic variation, was identified earlier on chromosome of the Indian rice landrace, Kasalath [9]. In search of novel sources of low soil P tolerance (i.e. other than Pup1), we earlier carried out the genotypic and phenotypic screening of Indian rice germplasm for low soil P. This effort culminated in the identification of a rice line from NorthEast part of India, named Wazuhophek, which showed excellent tolerance to low soil P and was devoid of Pup1 [3]. We designed and carried out this study intending to establish the genetic basis of tolerance to low soil P in Wazuhophek and undertake molecular mapping of tolerance associated loci using a recombinant inbred line mapping population developed from the cross between Wazuhophek (low soil P tolerant) and Improved Samba Mahsuri (low soil P sensitive)
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