Abstract
Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.
Highlights
Stem cells are a major component of regenerative medicine because of their potential to cure chronic diseases and regenerate organs [1]
As stated in the introduction, Polylactic Acid (PLA) was used in lieu of cellulosic
From 3D modeling in Solidworks it was calculated that the 30mm diameter lattice used has a theoretical surface area of 225cm2
Summary
Stem cells are a major component of regenerative medicine because of their potential to cure chronic diseases and regenerate organs [1]. They are defined by their abilities to self-renew and differentiate into functional cell types. Human Mesenchymal Stem Cell (hMSC) derived cell therapies are currently under clinical trials for cardiovascular, neurologic, bone and cartilage, lung, kidney, liver, and autoimmune diseases [3]. They show immunotolerant and immunomodulatory properties in allogenic transplants [2,4]. A possible solution is to culture the stem cells and produce large quantities through bioprocessing, yielding many therapeutic dosages from one harvest
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