Novel long noncoding RNA LINC01385 promotes nasopharyngeal carcinoma proliferation via the miR-140-3p/Twist1 signaling pathway

Abstract

ABSTRACT Long non-coding RNAs (lncRNAs) have been verified as a key modulator in tumor progression. However, the functions of lncRNAs in nasopharyngeal carcinoma (NPC) remain unclear. In the present study, we explored lncRNAs expression patterns in NPC tissues by GEO dataset and selected the high expression lncRNA (LINC01385) to further study. Our data showed that LINC01385 expression was significantly increased NPC and correlated with advanced clinical features and poor prognosis. Function assays showed that knockdown of LINC01385 expression reduced the proliferation and invasion abilities of NPC cells in vitro. In mechanism, LINC01385 acted as a molecular sponge of miR-140-3p in NPC cells, Twist1 mRNA was validated as a direct target of miR-140-3p in NPC cells. The effects of the LINC01385 knockdown on malignant characteristics of NPC cells were greatly attenuated by miR-140-3p inhibition or Twist1 overexpression. Thus, we illustrated that LINC01385 aggravated the progression of NPC by sponging miR-140-3p and upregulating Twist1 expression, which implied LINC01385 might serve as a new potential therapeutic target for NPC treatment.