Abstract
Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suis using real-time PCR
Highlights
As the importance of chlamydial infections in pigs has become more obvious, a rapid and sensitive method to study the prevalence of Chlamydia suis in pig herds is required
Chlamydia suis, Chlamydophila abortus, Chlamydophila psittaci and Chlamydophila pecorum infections have been identified in pigs
Using a locked nucleic acid (LNA)-containing probe, in this study, we evaluated a real-time PCR assay specific for C. suis
Summary
As the importance of chlamydial infections in pigs has become more obvious, a rapid and sensitive method to study the prevalence of Chlamydia suis in pig herds is required. Chlamydophila abortus, Chlamydophila psittaci and Chlamydophila pecorum infections have been identified in pigs. Chlamydial infections have been implicated in a wide spectrum of clinical disorders, including reproductive disorders, ocular and respiratory problems (e.g. conjunctivitis and pneumonia), enteritis, pleuritis, and polyarthritis [4]. The use of intensive pig production systems might increase the risk of chlamydial disease in pigs [6]
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