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Abstract
This study aimed at evaluating the therapeutic remineralising effects of innovative light-curable materials (LCMs) containing two experimental calcium silicate-based micro-fillers (TCS) modified with β-TCP only or β-TCP, zinc oxide (ZnO)/polyacrylic acid (PAA) on mineral-depleted bonded-dentine interfaces in simulated body fluids (SBFS). Three experimental LCMs were formulated: (1) resin A, containing a β-TCP-modified TCS (βTCS) micro-filler; (2) resin B, containing a polycarboxylated β-TCP/ZnO-modified TCS (βZn-TCS) micro-filler; and 3) resin C, containing no filler (control). Acid-etched (35% H3PO4) dentine specimens were bonded using the three LCMs and submitted to atomic force microscope (AFM)/nano-indentation analysis to evaluate the modulus of elasticity (Ei) and hardness (Hi) across the interface after SBFS storage (24 h/1 m/3 m). The ultramorphology and micropermeability of the resin–dentine interface were evaluated using confocal laser microscopy. Resin–dentine sticks were created and submitted to microtensile bond strength (μTBS) test (SBFS: 24 h/3 m). Scanning electron microscopy (SEM) was performed after de-bonding. The LCMs containing the experimental bioactive micro-fillers reduced the micropermeability and induced a significant increase of the Ei and Hi along the bonding interface. The specimens created using the resin B (βZn-TCS) attained the highest μTBS values both after 24 h and 3 m of SBFS storage. In conclusion, the innovative bioactive light-curable materials tested in this study are able to induce a therapeutic remineralising effect on the nano-mechanical properties and on the sealing ability of mineral-depleted resin–dentine interfaces. The contemporary idea of minimally invasive operative treatment, where therapeutic restorations are performed to combat the carious process and remineralise the dental hard tissues, may be satisfied by using such resin-base systems, containing βTCS or βZn-TCS bioactive micro-fillers.
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