Abstract
Novel Isoform Sequencing Based Full-Length Transcriptome Resource for Indian White Shrimp, Penaeus indicus
Highlights
Increasing the number of farmed species is one of the main strategies for enhancing aquaculture diversification toward sustainable production (Harvey et al, 2017)
Global shrimp production is dominated by Penaeus vannamei and Penaeus monodon which accounted for 82.7 and 12.5% of production, respectively (FAO, 2020)
Focussing on one or two species may be economically favorable in short-run owing to efficient technical advances made in limited number of species (Metian et al, 2020), dependence on single species might prove detrimental for long-term sustainable production
Summary
Increasing the number of farmed species is one of the main strategies for enhancing aquaculture diversification toward sustainable production (Harvey et al, 2017). For the first time, we have generated a transcriptome assembly for P. indicus to support the efforts of developing another shrimp species for aquaculture diversification and associated genetic improvement programs. We have used the latest Pacific Biosciences (Pacbio) Isoform Sequencing (Iso-Seq) approach on Sequel II instrument using 8M single molecule realtime (SMRT) sequencing cell and circular consensus sequence (CCS) technology to assemble transcriptome for P. indicus. This is the first report of a comprehensive transcriptome generated from different tissues and larval stage of P. indicus with 238.98 Gb of sequence data from gills, hepatopancreas, muscle, and pooled post-larvae. In the absence of a genome for P. indicus, the full-length transcriptome generated in this study would be a valuable resource for conducting functional studies involving desired economic traits
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
