Abstract
LncRNA play important roles in regulation of host immune and inflammation responses in defending bacterial infection. Clostridium perfringens (C. perfringens) type C is one of primary bacteria leading to piglet diarrhea and other intestinal inflammatory diseases. For the differences of host immune capacity, individuals usually show resistance and susceptibility to bacterial infection. However, whether and how lncRNAs involved in modulating host immune resistance have not been reported. We have investigated the expression patterns of ileum lncRNAs of 7-day-old piglets infected by C. perfringens type C through RNA sequencing. A total of 16 lncRNAs and 126 mRNAs were significantly differentially expressed in resistance (IR) and susceptibility (IS) groups. Many lncRNAs and mRNAs were identified to regulate resistance and susceptibility of piglets through immune related pathways. Five lncRNAs may have potential function on regulating the expressions of cytokines, these lncRNAs and cytokines work together to co-regulated piglet immune response to C. perfringens, affecting host resistance and susceptibility. These results provide valuable information for understanding the functions of lncRNA and mRNA in affecting piglet diarrhea resistance of defensing to C. perfringens type C, these lncRNAs and mRNAs may be used as the important biomarkers for decreasing C. perfringens spread and diseases in human and piglets.
Highlights
Diarrhea is one of the important reasons leading piglet death, causing the huge economic losses in worldwide pig industries, especially the newborn and suckling piglets[1]
In the process of bacterial infections, the severity of infection is impacted by pathogenicity of microbiota and its interaction with host immunity defense system[8]
After C. perfringens type C infection, a total of 359 lncRNAs and 2588 mRNAs were significantly expressed in the IR vs IC group (Supplementary Table S2, Fig. 2A), as well as 419 lncRNAs and 3283 mRNAs in the IS vs IC group (Supplementary Table S3, Fig. 2A)
Summary
Diarrhea is one of the important reasons leading piglet death, causing the huge economic losses in worldwide pig industries, especially the newborn and suckling piglets[1]. While chronic disease can persist more than one week, which is characterized by intermittent or persistent diarrhea with or without blood and dehydration It affects the growth and development of sick piglets, even stiff pigs and death[3]. An amount of differentially expressed lncRNAs and mRNAs were identified between resistant and sensitive piglets, which might play important roles in regulating piglet resistance to C. perfringens type C infection, though the potential roles of lncRNAs should be further validated. These results propose as a reliable model for exploring host resistance mechanisms in defensing C. perfringens type C infection, which may provide valuable foundation for further breeding diarrhea-resistance piglet strain
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