Novel insight into the formation mechanism of volatile flavor in Chinese fish sauce (Yu-lu) based on molecular sensory and metagenomics analyses

Abstract

Complex microbial metabolism is the basis for flavor formation in traditional fish sauce. To guide the targeted regulation of production quality, we used molecular sensory and metagenomics analyses to determine dynamic changes in volatile flavor compounds and microbial communities of fish sauce as fermentation progressed. In total, 56 volatile compounds were identified; of these, 3-methylthiopropanal had the highest average odor activity value. Twelve volatile compounds, key for fish sauce flavor development, were identified. Bidirectional orthogonal partial least squares analysis was applied to investigate the correlation between microorganisms and flavor substances. Five microbial genera including Halanaerobium, Halomonas, Tetragenococcus, Halococcus and Candidatus Frackibacter constituted the core microbial flora responsible for flavor formation. The microbial metabolic pathways degraded raw materials into primary metabolites, such as glucose, amino acids, and fatty acids. This study provides novel insights into the flavor formation mechanism of traditional fish sauce fermentation.