Novel insight into kinetin-inducible stress responses in rice seedlings

Abstract

Kinetin (KN) action in rice self-defense mechanism was studied using our established 2-week-old rice ( Oryza sativa L. japonica-type cv. Nipponbare) seedling in vitro model system. It was strikingly observed that KN caused formation of brownish necrotic microlesions in leaves, suggesting it triggers a stress response in rice. Subsequent northern analyses revealed differential regulation (both up-and down-regulations) of 10 prominent defense/stress-related marker genes, including the critical pathogenesis-related (PR) protein genes of class 1, 5 and 10. A systemic effect of KN in leaves was shown using OsPR1b (basic) and OsPOX (peroxidase) genes as representatives. KN also exclusively triggered potent accumulation of PR proteins (OsPR5 and OsPR10), and a phytoalexin, sakuranetin. Interestingly, as KN failed to induce jasmonic acid (JA) inducible genes ( OsPR1a and JIOsPR10), and had almost no effect on accumulated endogenous JA level due to wounding by cut, KN might act through a yet unknown (and JA-independent) pathway. These results provide a new aspect on the role of KN as a potent activator of the stress responses in the rice plant.