Abstract
Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil meta-genomic libraries. Soil environment has immense diversity of microorganisms, yet most remains unexplored and the metagenome are the technologies used and investigate uncultured microorganisms potential. The aim is to identify novel genes using the metagenomic approaches from a bioinformatics perspective and hopefully will serve as a useful resource. With this purpose, we used the metagenomic library of Eucalyptus spp. arboretum (EAA). We did a screening to select a positive clone and submitted them to the process of shotgun. The data obtained was submitted to bioinformatics analyses. These analyses identified were the novel MetaPPase gene and were classified according to the predict family and subfamily.
Highlights
Inorganic Pyrophosphatases (PPase, E.C 3.6.1.1), are ubiquitous and is the central enzyme of phosphorus metabolism [1]
We looked for a novel gene of PPase families in a soil metagenome, because we know that soil harbors an immense diversity of microorganisms yet most remains unexplored [5]
We work with a metagenomic library of a Eucalyptus spp. arboretum (EAA), belonging to Laboratory of Biochemistry and microorganisms of Plants (LBMP), to identify novel gene of PPase families activity, which was submitted to bioinformatics analyses, modeling for testing the biological potential
Summary
Inorganic Pyrophosphatases (PPase, E.C 3.6.1.1), are ubiquitous and is the central enzyme of phosphorus metabolism [1]. This enzyme is an important control of the cellular concentration inorganic pyrophosphate (PPi) and controlling biosynthetic reactions like nucleic acid and protein system and is responsible for the pump across the membranes responsible for triplicating ions [2]. The PPases are made up of two groups, Soluble PPases (Family I and II) and membrane PPase (M-PPase) [2]-[4] These groups of enzyme are very important for maintaining life. For this reason, we looked for a novel gene of PPase families in a soil metagenome, because we know that soil harbors an immense diversity of microorganisms yet most remains unexplored [5]. We work with a metagenomic library of a Eucalyptus spp. arboretum (EAA), belonging to Laboratory of Biochemistry and microorganisms of Plants (LBMP), to identify novel gene of PPase families activity, which was submitted to bioinformatics analyses, modeling for testing the biological potential
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