Novel Immunization Procedures Used to Develop Monoclonal Antibodies that Bind to Specific Structures inMeloidogynespp.

Abstract

Homogenates of the anterior regions of Meloidogyne incognita adult females injected into the spleen of Balb/c mice stimulated the production of nine monoclonal antibodies (MAbs) that bound to secretory granules formed in the nematode dorsal esophageal gland. Five of these nine MAbs were female-specific and bound to secretory granules in M. incognita, M. javanica, and M. arenaria, but not those in M. hapla or Heterodera glycines (...)