Abstract
Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material’s composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.
Highlights
IntroductionPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations
Fourier transform infrared spectroscopy (FTIR) provided an insight into structural characteristics of scaffolds based on alginate, gelatin, Hydroxyethyl methacrylate (HEMA), and HAp
Gelatin, HEMA, and HAp-based materials could be used for biomedical applications, we evaluated their toxicity in vivo using the zebrafish model
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. A major challenge in regenerative engineering is the design and fabrication of a suitable scaffold, which can mimic the native extracellular matrix (ECM) to regenerate functional tissues In this respect, hydrogels are one of the most promising biomaterials based on their high water content, biocompatibility, and easy tunability for recreating the properties of the ECM [4]. We set a hydrogel scaffolding biomaterials platform of porous scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, with and without inorganic agent hydroxyapatite, which was fabricated by cryogelation and characterized to show that the proposed technique of synthesis provides favorable mechanical, morphological, and degradation properties, as well as in vitro and in vivo suitable biocompatible behavior, which is crucial to provide a desirable environment for the viability and survival of cells. The effect of the composition of hydrogel scaffolds on the aforementioned properties was investigated as well
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
