Abstract
An excellent oil-water interface is one of the prerequisites for effective lipase catalysis. Therefore, this study aimed to improve lipase activity in terms of catalytic interface optimization. A novel approach for constructing oil-water interfaces was proposed. The structural similarity and the hydrophilic differences between polyvinyl pyrrolidone gel-fiber membranes (GFMs) and poly(lauryl methacrylate) (PLMA) organogel inspired us to hybridize the two to form PVP/PLMA hybrid gel-fiber membranes (HGFMs) based on electrospinning and gelation. The prepared PVP/PLMA-HGFMs were capable of being adopted as novel carriers for lipase catalysis due to their ability to swell both in the aqueous phase (swelling ratio = 187.5%) and the organic phase (swelling ratio = 40.5%). Additionally, Confocal laser scanning microscopy (CLSM) results showed that abundant network pores inside the carriers enabled numerous effective microscopic oil-water interfaces. The catalytic activity of Burkholderia cepacia lipase (BCL) in PVP/PLMA-HGFMs ranged between 1.21 and 8.70 times that of the control ("oil-up/water-down" system) under different experimental conditions. Meanwhile, PVP/PLMA-HGFMs increased lipase activity by about eight times at -20 °C and had good application characteristics at extreme pH conditions.
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