Novel HPLC method for simultaneous estimation of gallic acid, protocatechuic acid and mangiferin in mango

Abstract

Mango (Mangifera indica) is the main tropical fruit of Asia and has developed its prominence all over the world. Mango wastes such as peels and seeds generated from fruit are a good source of functional ingredients out of which important ones are phenolic compounds with potent antioxidant properties. The quantification of gallic acid (GA), protocatechuic acid (PCA) and mangiferin (MGF) in the seed and peel extracts of three different varieties of mango fruit was performed by a novel, simple, specific, linear, precise, accurate and economic RPHPLC (reversed-phase high- performance liquid chromatography) method. The chromatographic separation was achieved on the C18 analytical column with a flow rate of 1.2 mL/min in an isocratic mode using 0.2% orthophosphoric acid and acetonitrile in the ratio 90:10 as the mobile phase. The column temperature was maintained at 40 ℃ and observations were recorded at 242 nm. The retention time of gallic acid, protocatechuic acid and mangiferin was observed at 3.41, 5.50, and 14.6 min respectively. The developed method when validated as per ICH guidelines was found to be specific, linear, precise, robust and accurate