Abstract
Herein, we have reported a novel green approach for the biosynthesis of Co3O4/ZnO nanocomposite (CZNC) via Azadirachta indica (A. indica) aqueous leaf extract for the first time. In this method, we have used solutions of cobalt chloride and zinc acetate as a source of metal ions and A. indica green leaves’ extract as a bioreducing agent. Involvement of biomolecules of extract for reducing and stabilizing of nanostructures was confirmed by Fourier transform infrared (FTIR) spectroscopy. Scanning electron microscopy (SEM) revealed the formation of two different shape (spherical and hexagonal) nanostructures in the prepared sample which was further confirmed by TEM analysis. Element composition and uniform distribution of Co3O4 and ZnO was detected through Energy dispersive X-ray spectral (EDX) study. Powder XRD analysis revealed the polycrystalline nature of prepared sample with average particle size ∼34 nm. Outcomes of X-ray photoelectron spectroscopy illustrated the formation of Co3O4-ZnO heterojunction which was in agreement with XRD and TEM results. Further, biosynthesized and characterized CZNC were examined for antimicrobial efficacy against E. coli and P. chrysogenum. The results of biocidal activity indicated that Co3O4/ZnO nanocomposite possess more potential to inhibit E. coli compared to P. chrysogenum.
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