Abstract
A novel fractional purification approach of crude polysaccharides with a boronic acid-tagged thermoresponsive triblock copolymer PEG113-b-PVBA49-b-PNIPAM105 was designed in this paper. The fractional precipitation of polysaccharides was realized through simple temperature regulation based on the difference in the lower critical solution temperatures of the triblock copolymer–dextran complex. The precipitated polysaccharides from the extracting solution was in a descending order of molecular weight. Under optimal conditions, four astragalus polysaccharide fractions (APS1, APS2, APS3, and APS4) with narrow distribution of molecular weights were obtained with the temperature-controlled fractional precipitation. The structural properties, monosaccharide compositions, and molecular weights of APS1, APS2, APS3, and APS4 were characterized by the Fourier transform infrared spectrum, Congo red spectrophotometric method, gas chromatography, and gel permeation chromatography. The differences in the biological activ...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
