Abstract
An efficient fermentation-strengthening approach was developed to improve the anaerobic production of propionic acid and vitamin B12 by co-fermentation with Propionibacterium freudenreichii. Vitamin B12 production from glucose resulted in relatively high productivity (0.35mg/Lh) but a low propionic acid yield (0.55g/g). By contrast, glycerol gave a high propionic acid yield (0.63g/g) but low productivity (0.16g/Lh). Co-fermentation of glycerol and glucose with a gradual addition strategy gave high yields (propionic acid: 0.71g/g; vitamin B12: 0.72mg/g) and productivities (propionic acid: 0.36g/Lh; vitamin B12: 0.36mg/Lh). Finally, the integrated feedstock and fermentation system strengthening strategy was demonstrated as an efficient method for the economic production of bio-based propionic acid and vitamin B12.
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