Abstract
This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum. A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in the BCCM/LMG Bacteria Collection, Ghent University, Belgium by the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 104 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography–mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars—D-glucose and D-mannose—in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glcp-(1→3)-β-D-Manp-(1→6)-β-D-Glcp-(1→]n, which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications.
Highlights
Lactic acid bacteria (LAB) have attracted increasing attention in the food production of probiotics, owing to their benefits to human and animal health [1,2]
The growth of MC2, N9, and N10 strains under the cultivation conditions used generated poor EPS yields of 56.581, 58.939, and 69.508 mg/L, respectively
The results showed that the amount of EPS produced by the MC3 strain is significantly higher than that produced by the other strains (Figure 1)
Summary
Lactic acid bacteria (LAB) have attracted increasing attention in the food production of probiotics, owing to their benefits to human and animal health [1,2]. Many LAB strains have demonstrated exopolysaccharide (EPS) production capabilities. These EPSs exhibit good rheological properties, such as viscosity, emulsion stability, and gelling, and they act as prebiotics [1,2,7,8,9,10,11]. The identification methods include traditional [12] as well as molecular biotechnological methods, such as 16S rRNA sequencing [13,14,15], phenylalanyl synthetase (pheS) and ribonucleic acid (RNA) polymerase enzyme (rpoA) sequencing [16,17], repetitive extragenic palindromic sequence-based polymerase chain reaction (rep-PCR) fingerprinting [14,18], (GTG)5 -PCR [19], and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) [14,20,21]. The results of these methods are occasionally incompletely exact
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