Novel Epigallocatechin-3-Gallate (EGCG)-Loaded Mesoporous Bioglass Scaffolds for Bone Recruitment Applications

Shih-Fu Ou,Fang-Yu Fan,Liping Wang,Yuan-Li Tsao,Yi-Ting Wang,Wei-Chun Lin

doi:10.3390/app11010243

Abstract

Bioglass-based material has been widely used in the field of biomedical science. In this study, the proper concentration of epigallocatechin-3-gallate (EGCG) for a mesoporous bioglass (MBG) scaffold was determined based on the sponge replication method. The fabrication procedure performed using a foam exchange technique resulted in an interconnected network of pores scaffolds with no cracks. In the minimum bactericidal concentrations of the bacteria assessed, the antibacterial concentration of EGCG against E. coli (200 μg/mL) was higher than that against S. aureus (25 μg/mL). The MBG and EGCG-MBG scaffolds exhibited excellent apatite mineralization and drug release abilities (the highest cumulative drug release from the EGCG-MBG scaffold was 75.37%). Thus, a 200 μg/mL EGCG can prevent cell apoptosis and directly enhance cell proliferation. Hence, a low-dose EGCG-MBG scaffold is another option for bone recruitment material.

Highlights

Using suitable substitutes for damaged bone will improve the recruitment effect in clinical treatment
Bioceramics members that maintain the function of bone regeneration include bioglass, hydroxyapatite (HA), and tricalcium phosphate (TCP)
Such different types of bioceramics have been used to prove that Ca2+ and (PO4 )3− ions released from the surface of materials enhance apatite performance [1,2]

Summary

Introduction

Using suitable substitutes for damaged bone will improve the recruitment effect in clinical treatment. Bioceramics members that maintain the function of bone regeneration include bioglass, hydroxyapatite (HA), and tricalcium phosphate (TCP). Such different types of bioceramics have been used to prove that Ca2+ and (PO4 )3− ions released from the surface of materials enhance apatite performance [1,2]. The fabricating methods of porous ceramics include solvent-casting particle leaching, freeze-drying, and the foam exchange technique. These manufacturing processes can replicate the 3D porous structure in bone tissue engineering [4,5,6]. The foam exchange technique is a simple strategy which can provide a highly interconnected structure

Methods

Results

Conclusion