Abstract
A soft-lithography technique is used to fabricate a microfluidic device with very unique dome-shaped structures (see image), which are essential for high-efficiency immobilization and patterning of single beads. Immobilization of the microbeads was achieved by physical entrapment of the beads and patterning was performed at a relatively high flow rate. With such features, encoded microbeads can be incorporated into microfluidic devices to carry out biological assays, significantly increasing their multiplexing capability.
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