Abstract
The mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications.
Highlights
Resin composites and adhesive systems are increasingly popular for tooth cavity restorations [1,2].secondary caries at the restoration margins is considered as a main limitation of the longevity of the restorations [3]
Considering the increasing attention to the concept of minimally invasive cosmetic dentistry (MiCD), a new challenge to resin composites and adhesive systems is the possibility of a residual demineralization and a bacteria-containing layer in the cavity after preparation, which may cause a magnification of the problems associated with microleakage and lead to pulp inflammation [4]
The results indicated that the adhesive containing both poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) significantly inhibited lactic acid production and the growth of the three-species biofilms, the metabolic activity, and EPS synthesis
Summary
Resin composites and adhesive systems are increasingly popular for tooth cavity restorations [1,2]. Secondary caries at the restoration margins is considered as a main limitation of the longevity of the restorations [3]. Considering the increasing attention to the concept of minimally invasive cosmetic dentistry (MiCD), a new challenge to resin composites and adhesive systems is the possibility of a residual demineralization and a bacteria-containing layer in the cavity after preparation, which may cause a magnification of the problems associated with microleakage and lead to pulp inflammation [4]. Novel anti-caries adhesives with antibacterial and remineralization properties are needed. Materials 2017, 10, 26 lead to 2017, pulp, inflammation [4].
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