Abstract
Author(s): Samuelsen, GS; Rasmussen, RE; Nair, BK; Crocker, TT | Abstract: A novel method for exposing mammalian cells in vitro to air pollutants under conditions resembling those in vivo is described. Cells grown on membrane filters are nourished and protected from drying during exposure by perfusion of nutrient medium from the opposite surface of the filter. The cells are covered by a thin layer of fluid comparable in thickness to the mucus layer overlying the respiratory epithelium. Exposure to NO2 at levels commonly measured in the ambient air of urban settings (0.1-0.2 ppm) produces a pronounced cytotoxic effect after less than 4 h of pollutant encounter. It is proposed that this cell exposure system will have utility in the study of effects of airborne pollutants on mammalian cells. © 1978, American Chemical Society. All rights reserved.
Highlights
The in vitro analysis of the events surrounding the interaction of airborne pollutants and cells in culture, including cultured cells from respiratory epithelium, has been obliged to deal with unrealistically thick overlays of medium
Data are of limited value because of the inability t o maintain the cells in a state similar to that in vivo during the exposure to the pollutant
Does this appear to be relevant to the mode of injury that occurs in vivo, but this particular physical configuration of the in vitro system is necessary to produce data analogous to the in vivo response of respiratory epithelial cells
Summary
Long-term effects such as tumor development ( I ) and chronic lung disease ( 2 )may be shown in animal models These studies do not allow conclusions regarding the initial biochemical events that occur a t the first contact of cells and pollutants, and in addition, are complex and expensive to carry out. The problem is well illustrated by studies of the effect of NO2 on conventionally grown cell cultures [4].When covered by a few millimeters of serum-containing medium, the cells were resistant to killing by NO2 in concentrations up to 4100 ppm. The present paper reports on the development and testing of a cell culture and exposure system for studying monolayers of mammalian cells in vitro to air pollutants under conditions that closely resemble those in vivo
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
