Novel coumarin cyclotriphosphazene derivatives: Synthesis, characterization, DNA binding analysis with automated biosensor and cytotoxicity

Abstract

The synthesis of many new anti-cancer compounds is currently in drug discovery area, unfortunately the side effects of these compounds are not eliminated. In the efforts to develop suitable anticancer drugs, medicinal chemists have focused on coumarin derivatives. To understand the effects of the coumarin derivatives on DNA, it is necessary to analyze the interaction with in vitro tests due to DNA is a target molecule for anticancer drug development and investigation on DNA interactions with synthesized potential compounds. Electrochemical biosensors provide good alternative for the quantitative analysis of DNA/compound interactions. In this study, we have prepared and characterized tetra novel coumarin substituted cyclotriphosphazene derivatives (3–6). By expanding our study, we performed biological activity studies of all compounds together with the compounds our synthesized before (7-12). All compounds were characterized by elemental analysis, MALDI-TOF mass spectrometry, FT-IR, 1H and 31P NMR techniques and screened to effect on DNA using an automated biosensor device. Compounds were also evaluated for their anti-cancer potential against cancer cell lines to assess whether these derivatives may serve as a lead for the augmentation of anti-cancer drug. All compounds were assessed for cytotoxicity by the MTT assay against two different cancer cell lines, MCF-7 and DLD-1 and most of these compounds have cytotoxic effects for MCF-7 and DLD-1 cells.