Abstract
A new approach for the preparation of adsorbents for one-step isolation/purification of DNA from different samples (e.g., bacterial lysates, smears and blood) has been developed. The technique is based on the use of porous silica preliminary treated with ozone followed by grafting of polymer nanolayers on the activated carrier surface. The chemical nature of active centers, their stability and conditions for the use of the activated support as heterogeneous initiator of different macromolecular reactions were studied. The adsorbents modified with thin (~3.0-7.5 nm) polytetrafluoroethylene and polyaniline layers were prepared and characterized. Sorption properties of the obtained adsorbents were demonstrated on examples of express (2-5 min) one-step DNA isolation for direct use in PCR diagnostics. The effectiveness of the developed adsorbents used for DNA isolation and purification was confirmed in comparison with the standard methods. Thus, a facile (alternative in relation to irradiation postpolymerization or standard oxidative polymerization techniques) manufacturing method of the materials for nucleic acids sample preparation was developed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
