Abstract
Purpose: To develop a novel colorimetric assay method for mefenamic acid capsules. Method: The new method (aromatic ring derivatization technique) is based on a diazo coupling reaction using diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA) as a chromogenic derivatizing reagent. Result: Optimization studies showed that the coupling reaction is very fast and completed in less than 1 minute. A 1:1 drug to reagent stoichiometric ratio was obtained for the azo dye formed. The azo adduct formed exhibits bathochromic shift with absorption maximum (lmax) at 490 nm, which was selected as the analytical wavelength. Lower limit of quantitation of mefenamic acid was 1 mg/ml. The assays were linear over the concentration range of 1 - 6 mg/ml and reproducible. This new method has been successfully applied in the assay of mefenamic acid capsules with accuracy similar to the official (B.P) titrimetric method of assay (p>0.05) and has the advantages of speed, high sensitivity, lower limit of detection and can be automated. Conclusion: The method developed could find application in in-process quality control of mefenamic acid capsules. Keywords: Mefenamic acid assay, 4-amino-3,5-dinitrobenzoic acid, colorimetry, diazotization Trop J Pharm Res, June 2002; 1(1): 15-22
Highlights
Mefenamic acid is a non-steroidal antiinflammatory drug (NSAID) which has analgesic, anti-inflammatory and antipyretic actions. It is been used for the relief of mild to moderate pain. It is indicated for the treatment of rheumatoid arthritis[1], primary dysmenorrhea[2] and periodontitis[3]
Determination in urine was reportedly done by HPLC9
Content of the mefenamic acid capsules were determined by interpolation from calibration lines
Summary
Mefenamic acid is a non-steroidal antiinflammatory drug (NSAID) which has analgesic, anti-inflammatory and antipyretic actions. It is been used for the relief of mild to moderate pain. It is indicated for the treatment of rheumatoid arthritis[1], primary dysmenorrhea[2] and periodontitis[3]. Determination in the serum has been carried out with high performance liquid chromatography (HPLC) with UV detection and spectrofluorimetry[7]. Determination in the blood has been carried out with HPLC with photodiode array detector and gas chromatography (GC) with flame ionisation detector[8].
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