Novel Circular Rep-Encoding Single-Stranded DNA Viruses Detected in Treated Wastewater.

Karyna Rosario,Kaitlin A Mettel,Walter Q Betancourt,Christina M Morrison

doi:10.1128/mra.00318-19

Abstract

Here, we present the complete genome sequences of three circular replication-associated protein (Rep)-encoding single-stranded DNA (CRESS DNA) viruses detected in secondary treated and disinfected wastewater effluent. The discovered viruses, named wastewater CRESS DNA virus (WCDV)-1 to -3, represent novel viral species that seem to persist in wastewater effluent.

Highlights

We present the complete genome sequences of three circular replication-associated protein (Rep)-encoding single-stranded DNA (CRESS DNA) viruses detected in secondary treated and disinfected wastewater effluent
The wastewater CRESS DNA virus (WCDV) contain a putative origin of replication marked by a conserved nonamer (TAGTATTAC) at the apex of a predicted stem-loop structure (5)
No Yes No a nt, nucleotides. b Best BLAST match based on BLASTx searches using the replication-associated protein (Rep)-coding sequence as a query. c Rep amino acid/genome-wide sequence identities between WCDVs and their best BLAST match based on the Rep

Summary

Introduction

We present the complete genome sequences of three circular replication-associated protein (Rep)-encoding single-stranded DNA (CRESS DNA) viruses detected in secondary treated and disinfected wastewater effluent. Three CRESS DNA viruses, named wastewater CRESS DNA virus (WCDV)-1 to -3, were recovered from treated effluent (Table 1). Each genome was de novo assembled, with at least 3ϫ coverage, from 10 to 16 reads in Geneious version R7 (Biomatters), using default parameters, and nonoverlapping open reading frames longer than 300 nucleotides were identified in SeqBuilder version 11.2.1 (Lasergene package; DNAStar). Similar to known CRESS DNA viruses (5), the WCDV genomes encode the Rep and a capsid protein within a small circular DNA molecule.

Results

Conclusion