Abstract
The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP (%). The optimum cultivation conditions for obtaining the best FPase (600mU/ml) at 72h are 150rpm, 35°C, pH7, 0.2% soy peptone and 0.5% α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135mU/ml of FPase after 6days cultivation at pH5, D. caldariorum 263 grew faster at 35°C and produced the maximum FPase within 3days at pH7.
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