Abstract
The increase in global warming has favored growth of a range of opportunistic environmental bacteria and allowed some of these to become more pathogenic to humans. Aeromonas hydrophila is one such organism. Surviving in moist conditions in temperate climates, these bacteria have been associated with a range of diseases in humans, and in systemic infections can cause mortality in up to 46% of cases. Their capacity to form biofilms, carry antibiotic resistance mechanisms, and survive disinfection, has meant that they are not easily treated with traditional methods. Bacteriophage offer a possible alternative approach for controlling their growth. This study is the first to report the isolation and characterization of bacteriophages lytic against clinical strains of A. hydrophila which carry intrinsic antibiotic resistance genes. Functionally, these novel bacteriophages were shown to be capable of disrupting biofilms caused by clinical isolates of A. hydrophila. The potential exists for these to be tested in clinical and environmental settings.
Highlights
Aeromonas hydrophila is a Gram-negative rod found in fresh water, brackish water, and mud in temperate climates (Batra et al, 2016)
Five clinical isolates identified as A. hydrophila were screened for antimicrobial resistance genes (ARGs) by PCR amplification, revealing intrinsic multi-antibiotic resistance
Isolation of Novel Bacteriophages Against Clinical Strains of Aeromonas hydrophila Wastewater and pond samples collected from the cities of Bendigo and Melbourne, Victoria, Australia were screened for bacteriophages
Summary
Aeromonas hydrophila is a Gram-negative rod found in fresh water, brackish water, and mud in temperate climates (Batra et al, 2016). They are an established fish pathogen causing septicemia and ulcerative diseases (Chowdhury et al, 1990). Clinical infections may be a result of direct skin invasion from muddy water (Vally et al, 2004) or drinking contaminated water leading to local (gastritis, skin necrotizing infection) or systemic (peritonitis, sepsis, meningitis, respiratory, and hepatic) infections (Janda and Abbott, 2010; Igbinosa et al, 2012). Mortality in systemic infections may be as high as 46% (Dryden and Munro, 1989)
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