Abstract
This study describes an innovative approach for recovery and concentration of sesame glucosides from defatted sesame cake using membrane separation route. Simple aqueous extraction of the seed meal at suitable pH value followed by two steps membrane fractionation (ultrafiltration-nanofiltration) was done to obtain sesame glucoside concentrate. In this process, about 0.7% ± 0.29 recovery of total meal glucoside was achieved along with coproduct sesame protein isolate at yield value of 36.50%. Several in vitro analyses were carried out to assay the antioxidative potential of recovered glucosides in different reaction models. Recovered glucosides have shown comparable α,α′-diphenyl-1-picryl-hydrazyl radical scavenging activity and Cu2+ ion chelation capacity with solvent extracted glucosides. Sesame glucosides have shown unique antioxidant activity for 29 h in an induced oxidation system of linoleic acid emulsion. This study includes a detail analysis on membrane performance parameters and fouling characteristics of ultrafiltration membrane, which are crucial factors to consider for industrial relevance. All findings point toward the potentiality of ultrafiltration-nanofiltration-based technique for recovery of polar antioxidants from various raw materials.
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