Abstract
In this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis. The in vitro and in vivo biological potential of NRLP were evaluated by measuring its antioxidant (•OH and DPPH• scavenging, total antioxidant capacity), anti-α-amylase as well as anti-inflammatory and antinociceptive activities in a mice model. NRLP was composed of Rha (33.7%), Gal (18.1%), GalA (15.0%), Glc (13.3%), Ara (13.3%), Xyl (3.8%), and GlcA (2.8%) and showed a Molecular Weight (Mw) of 23.0 kDa and a polydispersity index (PDI) of 1.66. The investigations highlighted a significant antioxidant activity (IC50 = 2.4–2.6 mg/mL) and an inhibition activity against α-amylase (IC50 = 4.55 mg/mL) in a dose-dependent manner. Further, NRLP revealed interesting anti-edematous effects and antinociceptive activities (both > 70%). These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves.
Highlights
Inflammation, a physiological feedback to tissue injury or surgical trauma, requires the participation of various inflammatory mediators, including histamine, nitric oxide, serotonin, interleukin, prostaglandins, and tumor necrosis factors, as well as a variety of cell populations [1].Several papers have shown a relationship between oxidative damage, inflammation, and pain through the modulation of several immune cells and inflammatory mediators [2,3]
These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves
NRLP was obtained from N. retusa leaves
Summary
Inflammation, a physiological feedback to tissue injury or surgical trauma, requires the participation of various inflammatory mediators, including histamine, nitric oxide, serotonin, interleukin, prostaglandins, and tumor necrosis factors, as well as a variety of cell populations (macrophages and neutrophils) [1]. Several papers have shown a relationship between oxidative damage, inflammation, and pain through the modulation of several immune cells and inflammatory mediators [2,3]. The authors reported that the agents or substances that counteract oxidative stress could help to alleviate inflammatory disorders. Due to safety concerns associated with their use, research on new natural products, such as plant-derived polysaccharides, for the management of antinociceptive and anti-inflammatory disorders is of utmost importance [4,5,6].
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