Novel Antioxidant and Hypoglycemic Water-Soluble Polysaccharides from Jasmine Tea.

Yayuan Tang,Bo Lin,Guoming Liu,Li Li,Jian Sun,Changbao Li,Zhen Wei,Xuemei He,Jinfeng Sheng

doi:10.3390/foods10102375

Yayuan Tang, Bo Lin + Show 7 more

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https://doi.org/10.3390/foods10102375

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Abstract

There have been few studies dealing with chemical elucidation and pharmacological potentials of water-soluble polysaccharides from jasmine tea, limiting their use in functional foods. In this study, water-soluble polysaccharides (named as JSP) were extracted from Jasminum sambac (L.) Aiton tea and fractionated to afford two sub-fractions (JSP-1 and JSP-2). The main structural characteristics of novel JSP sub-fractions were determined by high performance gel permeation chromatography, ultra-performance liquid chromatography-tandem mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. Physiologically, the abilities of JSP-1 and JSP-2 to reduce ferric ions, scavenge DPPH and hydroxyl radicals, as well as protect islet cells were confirmed in vitro. JSP-1 exhibited better antioxidant and hypoglycemic activities than JSP-2. The molecular weights of JSP-1 and JSP-2 were 18.4 kDa and 14.1 kDa, respectively. JSP-1 was made up of glucose, galactose, rhamnose, xylose, arabinose, and galacturonic acid with molar ratios 1.14:4.69:1.00:9.92:13.79:4.09, whereas JSP-2 with a triple helical structure was composed of galactose, rhamnose, xylose, arabinose, and galacturonic acid as 3.80:1.00:8.27:11.85:5.05 of molar ratios. JSP-1 contains →1)-α-Galƒ-(3→, →1)-α-Galƒ-(2→, →1)-α-Araƒ-(5→, →1)-α-Araƒ-(3→, →1)-α-Araƒ-(3,5→, →1)-β-Xylp-(2→ and →1)-β-Xylp-(3→ residues in the backbone. These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from jasmine tea.

Highlights

Diabetes mellitus, as the third most life-threatening illnesses worldwide, is a syndrome of endocrine metabolic disorder characterized by sustained hyperglycemia
In view of the above facts, this paper further provides a reference for the potential utilization of jasmine tea polysaccharides in food industries
The crude Jasminum sambac flower polysaccharides (JSP) was purified by diethylaminoethyl 52 (DEAE-52) cellulose column eluted with ultra-pure water and different concentrations of NaCl, i.e., 0.1, 0.3, and 0.5 M

Summary

Introduction

As the third most life-threatening illnesses worldwide, is a syndrome of endocrine metabolic disorder characterized by sustained hyperglycemia. The high concentration of free radicals induced by chronic hyperglycemia can damage biological macromolecules in the islet cells and the pancreatic tissues, increasing the risk of diabetes. Protecting the islet cells from oxidative stress appears to be a crucial factor to lower blood glucose and successfully prevent diabetes [1]. Many edible and medicinal polysaccharides attract attention based on their special physico-chemical properties and physiological functions, including antioxidant and hypoglycemic activities [2]. The particular bioactivity of polysaccharides is linked to structural properties such as molecular weight, monosaccharide content, functional group and three-dimensional conformation, as well as glycosidic linkage [3]. The genus Jasminum belonging to the Oleaceae family is a kind of bulb flower with high potentials [4]. Jasminum sambac (L.) Aiton is mainly cultivated in tropical regions in

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