Novel Antihypertensive Peptides Derived from Chicken Foot Proteins.

Begoña Muguerza,Anna Mas‐Capdevila,Maria Margalef,Francisca I Bravo,Anna Arola‐Arnal

doi:10.1002/mnfr.201801176

Abstract

ScopeChicken foot proteins have recently been demonstrated by the group to be a great source of hydrolysates with antihypertensive properties. The aim of this study was to isolate and identify angiotensin I‐converting enzyme inhibitory (ACEI) peptides from chicken foot hydrolysate Hpp11 and to test their antihypertensive properties.Methods and ResultsPeptides are separated into fractions according to their molecular size and hydrophobicity by ultrafiltration and RP‐HPLC, respectively. Subsequent peptide identification in the two fractions that present the highest ACEI activities is carried out by HPLC‐MS. Ten of the identified peptides are synthesized and five of them show ACEI (IC50) values lower than 100 µm. The antihypertensive effects of these ACEI peptides after oral administration is evaluated in spontaneously hypertensive rats. The peptides AVFQHNCQE and QVGPLIGRYCG exhibit antihypertensive activity when administered at an oral dose of 10 mg kg−1 body weight. The maximal decrease in systolic blood pressure is recorded 6 h after their administration (−25.07 ± 4.21 and −10.94 ± 1.96 mmHg, respectively).ConclusionThese results suggest that AVFQHNCQE and QVGPLIGRYCG could be used as functional ingredients with antihypertensive effects, although it would be necessary to perform bioavailability and clinical studies to demonstrate their efficiency in humans.

Highlights

The maximal tem being angiotensin I-converting endecrease in systolic blood pressure is recorded 6 h after their administration (−25.07 ± 4.21 and −10.94 ± 1.96 mmHg, respectively). These results suggest that AVFQHNCQE and QVGPLIGRYCG could be used as functional ingredients with antihypertensive effects, zyme (ACE, EC 3.4.15.1) the key enzyme in this system
Hpp11 was first subjected to ultrafiltration through 3 kDa and 10 kDa cut-off membranes to separate the peptides according to their molecular size
The ACEI activity of the

Summary

Introduction

Universitat no 1, Reus, 43204, Spain ever, the administration of synthetic ACE inhibitors, such as captopril, lisinopril, and enalapril, has been reported to have adverse side effects.[5] there has been an increasing interest in development of the natural antihypertensive compounds and ACE inhibitors as alternative for lowering BP.[6] In this sense, bioactive peptides from dietary proteins have reported a wide range of biological activities,[7,8] including antihypertensive effect.[9,10,11] These peptides are enclosed in the native structure of the proteins and a treatment is needed to release these bioactive sequences.[12] other factors such as the possible structural changes performed during protein di-.

Objectives

Results

Conclusion