Abstract
Two novel antifungal peptides, designated α- and β-basrubrins, respectively, were isolated from seeds of the Ceylon spinach Basella rubra. The purification procedure involved saline extraction, (NH4)2SO4 precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose and FPLC-gel filtration on Superdex peptide column. α- and β-basrubrins exhibited a molecular weight of 4.3 and 5 kDa, respectively. They inhibited translation in a rabbit reticulocyte system with an IC50 value of 400 and 100 nM, respectively. α- and β-basrubrin inhibited HIV-1 reverse transcriptase by (79.4 ± 7.8)% and (54.6 ± 3.6)%, respectively, at a concentration of 400 μM, and (10.56 ± 0.92)% and (2.12 ± 0.81)%, respectively, at a concentration of 40 μM. Both α- and β-basrubrins exerted potent antifungal activity toward Botrytis cinerea, Mycosphaerella arachidicola, and Fusarium oxysporum.
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