Abstract
A glucose biosensor is fabricated with immobilization of glucose oxidase (GOx) on poly(pyrrole propylic acid)/Au nanocomposite by covalent attachment. Poly(pyrrole propylic acid) (PPyAA)/Au nanocomposite was prepared by chemical oxidation of pyrrole propylic acid monomer by using chloroauric acid (HAuCl4) as an oxidizing agent. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 0.42μA/mM, response time ∼2s, linear dynamic range from 1 to 18mM, correlation coefficient of R2=0.9981, and limit of detection (LOD), based on S/N ratio (S/N=3) of 0.05mM. A value of 1.83mM for the apparent Michaelis–Menten constant Kmapp was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.
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