Abstract
Zn2+-guar gum/pyruvic acid sodium (named as Zn-GG/PAS) composite hydrogel with chromogenic substrates 5-bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP-2Na) and 4-nitrophenyl palmitate (PNP-PAL) was synthesized by incorporating Zn2+ into guar gum/pyruvic acid sodium composite hydrogel and used for mold and yeast biochemical signal fluorescence and visible exhibition. Zn-GG/PAS exhibited excellent water holding capacity (95%) with a final concentration of Zn2+ of 0.1 g/L. When the concentration of Zn2+ was 0.1 g/L, the cohesiveness and springiness were 0.14 g and 2.9 g, respectively. After GG/PAS with composite ratio of 8/2 (w/w) was added to Zn-GG/PAS, the elasticity modulus and viscous modulus remained relatively stable with the scanning range of viscosity. The cross-linked network of Zn-GG/PAS hydrogel provided an excellent encapsulation efficiency of 94% until 120 h. Compared with the national standard GB 4789.15–2016, the alkaline phosphatase/lipase-responsive Zn-GG/PAS hydrogel had no significant difference in terms of mold and yeast colony count. This work provides a convenient and effective method for fabricating composite hydrogel with alkaline phosphatase and lipase stimulate responsive that has promising application to sensitive exhibition of mold and yeast biochemical signals in microbiological detection.
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